Rapid and Validated Hplc Assay for the Determination of Oxytetracycline in Biological Material

نویسنده

  • CEZARY KOWALSKI
چکیده

A rapid, accurate, simple, and reproducible high performance liquid chromatographic (HPLC) method for the determination of oxytetracycline hydrochloride (OTC) in porcine plasma has been developed and validated. The drug and the standard were eluted from 5 μm Omni Spher (Varian) C18 column (250 mm × 4.6 mm I.D.) at room temperature. The mobile phase was composed of ACN-MeOH-(HCOO)2 (17.5:17.5:65 v/v/v) (pH adjusted to 2). A flow rate was 1.4 mL/min. The effluent was monitored using a UV-VIS detector set at 360 nm. The retention time of OTC was about 3 min. The suggested technique is characterised by superior performance parameters: linearity R = 0.9999, recovery = 92.50%, repeatability RSD ≤ 1.39%. These results demonstrate the validity of the HPLC method for the analysis of OTC HCL.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

متن کامل

Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma

A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150?4 mm) and a UV detector (?= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phas...

متن کامل

Determination of mirtazapine and desmethyl mirtazapine in human plasma by a new validated HPLC ultraviolet method with a simple and reliable extraction method: Application to therapeutic drug monitoring study by 62 real patient plasma

Determination of mirtazapine during psychopharmacotherapy in biological fluids is essential to achieve successful therapy, to avoid toxicity related to drug interactions, genetic variability and poor compliance. A new, rapid and sensitive high-performance liquid chromatography method has been developed in human plasma for the determination of mirtazapine (MRP) and N-desmethylmirtazapine (NDM) t...

متن کامل

Determination of mirtazapine and desmethyl mirtazapine in human plasma by a new validated HPLC ultraviolet method with a simple and reliable extraction method: Application to therapeutic drug monitoring study by 62 real patient plasma

Determination of mirtazapine during psychopharmacotherapy in biological fluids is essential to achieve successful therapy, to avoid toxicity related to drug interactions, genetic variability and poor compliance. A new, rapid and sensitive high-performance liquid chromatography method has been developed in human plasma for the determination of mirtazapine (MRP) and N-desmethylmirtazapine (NDM) t...

متن کامل

RP-HPLC method development and validation for determination of eptifibatide acetate in bulk drug substance and pharmaceutical dosage forms

A new, rapid, economical and isocratic reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of eptifibatide acetate, a small synthetic antiplatelet peptide, in bulk drug substance and pharmaceutical dosage forms. The developed method was validated as per of ICH guidelines. The chromatographic separation was achieved isocratically on C18 colum...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2007